Fig. 6

LPS does not affect Panx 1 expression in cultured Schwann cells. A Panx 1 mRNA expression levels was determined by qPCR in LPS-treated Schwann cells (0, 100, 500, and 1000 ng/ml, 24 h at 37 °C). B–D Determination of Panx 1 mRNA and protein levels at 0 h, 12 h, and 24 h after LPS (100 ng/ml) treatment in Schwann cells. E, F The determination of Panx 2 and Panx 3 mRNA levels via qPCR at 24 h in post-LPS (100 ng/ml) treated Schwann cells. Each experiment was performed in triplicate. Data are presented as mean ± SEM