Fig. 7

LPS induces glycolytic pathway activity in acute hippocampal slices. Glucose uptake was measured by a radioactivity assay. Glucose and lactate medium levels were evaluated by a spectrophotometric method. Pyruvate carboxylase and PFK1 activities were analyzed by kinetic assay. Protein expressions of the glucose transporter (GLUT1) and phosphorylation of p38 MAPK and Akt were analyzed by Western blot. Gene expressions of the enzymes G6PD and AMPK were evaluated by RT-PCR. Inflammatory induction increased glucose uptake in hippocampal slices (A) and lactate extracellular levels (B). There was also a time-dependent release of lactate to the extracellular medium and a decrease of glucose content in the medium (C). LPS increases PFK1 activity (D), without changing pyruvate carboxylase activity (E) or changing GLUT1 transporter protein expression (F). Representative image of Western blot (G). Early inflammation induces phosphorylation of key enzymes related to energy metabolism, p38MAPK and Akt (H). The gene expression of G6PD was not affected (I), rather AMPK gene expression was downregulated (I). Data were analyzed by Student's unpaired t test, assuming P < 0.05. * means significant increase, when compared to sham group (* P < 0.05, ** P < 0.01), # means significant decrease