Fig. 6
From: ISGylation is induced in neurons by demyelination driving ISG15-dependent microglial activation
Cortical and deep gray matter in MS exhibit neuronal ISGylation. Paraffin-embedded MS postmortem brain tissue was obtained from Mayo Clinic tissue registry, Netherlands Brain bank, and Normal Aging Brain Collection Amsterdam (controls). Tissues were deparaffinized and antigen retrieved in 10 mM Tris 1 mM EDTA pH9.0 for 20 min at 95 C. Neuronal ISG15 immunostaining (brown) in cortical gray matter (A, B) and deep gray matter (C, D) from normal aged controls and MS patients. Representative micrographs shown in A and B. Qualitative staining scores are shown in B and D. Tissues were counterstained with hematoxylin (blue). Representative micrographs are shown. Insets are digitally magnified. **P < 0.01 by unpaired Student’s t-test. Scale bar = 100 microns. Mean ± SEM are shown