Fig. 1

scRNA-seq reveals altered retinal heterogeneity in mice with I/R injury. A Clustering strategy of major retinal cell populations identifying 12 cell types (left) based on the scaled expression heatmap of canonical markers for each cluster (right). Color scheme is based on z-score distribution from − 2 (blue) to 2 (red). B Relative changes in cell ratios in different cluster between Blank and I/R groups. The numbers on the right indicate the Log₂FC values of the cell ratios. C Violin plots showing the proportion of 8 clusters in retinal cells between Blank and I/R groups (n = 3/group). P value were calculated using a Wilcoxon rank-sum test. D t-SNE distribution showing I/R-upregulated DEGs numbers from 12 clusters. E GO and pathway enrichment analysis of I/R-upregulated DEGs in 12 clusters of the I/R model. P value was derived by a hypergeometric test