Fig. 2

I/R injury induced an expansion of photoreceptor subclusters with ferroptosis. A t-SNE distribution showing 4 subclusters in CONE. B t-SNE distribution showing groups (Blank and I/R) in CONE. C Bar plots showing cell abundances across CONE-SCs (n = 4) for the Blank and I/R groups. D Violin plots showing the proportion of CONE-SC3 in CONE between Blank and I/R groups (n = 3/group). P value were calculated using a Wilcoxon rank-sum test. E Heat map representing the scaled expression values of the top 10 genes defining each CONE-SCs. F t-SNE distribution showing 4 subclusters in ROD. G t-SNE distribution showing groups (Blank and I/R) in ROD. H Bar plots showing cell abundances across ROD-SCs (n = 4) for the Blank and I/R groups. I Violin plots showing the proportion of ROD-SC2 in ROD between Blank and I/R groups (n = 3/group). P value were calculated using a Wilcoxon rank-sum test. J Heat map representing the scaled expression values of the top 10 genes defining each ROD-SCs. K GO and pathway enrichment analysis of the CONE-SCs clustering DEGs. P value was derived by a hypergeometric test. L GO and pathway enrichment analysis of the ROD-SCs clustering DEGs. P value was derived by a hypergeometric test. M Violin plots showing the inflammatory response score between Blank and I/R groups among CONE (up) and ROD (down) clusters