Fig. 6

Epigenetic marking that inhibits neurogenesis after OHCs is repopulated with new neurons in advanced TD. The trimethylation of histone H3 lysine 36 (arrows in B) positively regulates the expression of the Bmp4 gene, an important inhibitor of neurogenesis. A Sum of the fluorescence intensity of H3K36me3 normalized by the number of mature neurons identified with this marking after 9 days of culture with or without B1. The medians of the CT (controls) and TD (thiamine deficiency) groups were compared with the Mann–Whitney test and data were expressed as median ± interquartile. (*P ≤ 0.05). B Confocal microscopy images (×100) of OHCs labeled for mature neurons (NeuN+) in red, cell nucleus (DAPI) in blue and H3K36me3 in green after 9 days of culture with or without B1 (scale bar = 50 μm)