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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: GSK872 and necrostatin-1 protect retinal ganglion cells against necroptosis through inhibition of RIP1/RIP3/MLKL pathway in glutamate-induced retinal excitotoxic model of glaucoma

Fig. 8

GSK872 and Nec-1 prevented glutamate-induced oxidative stress in R28 cells. a DCFH-DA probe was used to detect the intracellular ROS levels in R28 cells. The green fluorescence intensity indicated the level of intracellular ROS. Compared with the Glu + DMSO group, the fluorescence intensity was apparently decreased in the cells treated with GSK872 or Nec-1. Scale bar = 50 μm. b Flow cytometry was used to detect the DCFH-DA fluorescence density in R28 cells. c GSH levels in R28 cells were measured after administration of GSK872 or Nec-1. CTL: the control group; Glu: glutamate. The results were recorded as mean ± SD from at least three independent experiments. **p < 0.01, ***p < 0.001, ****p < 0.0001 versus control group; #p < 0.05, ##p < 0.01, ###p < 0.001 versus Glu + DMSO group

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Journal of Neuroinflammation

ISSN: 1742-2094