Fig. 3

Trem2 ablation results in reduced cellular proliferation and increased apoptosis within demyelinated lesions. A Representative images of cellularity in SC demyelinated lesions from WT and Trem2^−/− shown by FM (red) and DAPI (green) staining at days 14 and 20 pi. Scale bar = 200 μm. B Counts of total DAPI positive cell numbers measured in the white box (319.45 μm × 319.45 μm) of (A). C Representative images of anti-Ki67 mAb (green) and DAPI (blue) staining. Scale bar = 50 μm. D Counts of Ki67⁺ cells per mm². E, F Representative images, number and proportion of pyknotic nuclei per mm² demyelinated lesion area from WT and Trem2^−/− mice. Arrow points depict pyknotic nuclei. G Representative images of active-caspase 3 (green) and DAPI (blue) staining. Scale bar = 50 μm. H Counts of active-caspase 3⁺ cells per mm². Data show the mean ± SEM from 12 to 18 lesions from 3 mice (4–6 lesions/mouse) per timepoints and are analyzed by unpaired two-tailed Student t test and two-way ANOVA analysis followed by Bonferroni test. *p < 0.01, **p < 0.001