Fig. 6

Trem2 ablation impairs uptake and degradation of myelin debris following virus-induced demyelination. A, B Representative images of dMBP (red), CD68 (green) and DAPI(blue) staining in SC demyelinated areas of WT and Trem2^−/− mice at days 14 and 20 pi. Scale bar = 50 μm. C, D Quantification of volume of dMBP positive area (C) and CD68 positive cells (D) per mm³. E, F Representative images of co-staining for dMBP (red), IBA1 (white) and CD68 (green) in lesions of WT and Trem2^−/− mice at days 14 and 20 pi. Scale bar = 50 μm. a–c Zoomed-in images of boxed areas in (F). Representative images of ‘free’ dMBP (asterisk), dMBP associated with only IBA1 (open arrow) and dMBP associated with both IBA1 and CD68 (white arrowhead). Scale bar = 20 μm. Data show the mean ± SEM from 12 to 18 lesions from 3 mice (4–6 lesions/mouse) per timepoint and are analyzed by unpaired two-tailed Student t test and two-way ANOVA analysis followed by Bonferroni test. **p < 0.001