Fig. 4

TMEM119 expression within the DVC of control and DON-treated animals. A Representative photomicrographs of TMEM119 and IBA1 labeling taken from coronal brainstem sections of mice treated with vehicle or DON. The white lines symbolize the border between AP and NTS. B Representative images of double TMEM119/IBA1 labeling highlighting the phenotype of microglial cells located within the AP and the funiculus separans of control mice. The white box indicated regions from panels A where panel B originated. C Quantification of TMEM119 labeling expressed in mean gray level in subregions of the DVC of control and DON-treated animals (12.5 mg/kg BW; *p < 0.05). Scale bars: 500 µm in A, 10 µm in B. AP area postrema; cc central canal; fs funiculus separans; NTS nucleus tractus solitarius