Fig. 8

Neuronal expression of osteoprotegerin is upregulated by nuclear calcium signaling and neuronal activity in TBI. A, B Significant increase of OPG (TNFRSF11b) mRNA density 3 h post-TBI compared to sham (CS vs CT; 8.78 ± 1.95 vs 18.03 ± 2.08). Buffering of nuclear calcium suppresses the upregulation OPG mRNA upon TBI (CT vs PT; 18.03 ± 2.09 vs 11.06 ± 2.80). Small datapoints depict individual neurons, red datapoints depict average per animal (N = 4, used for statistics). C, D Chemogenetic inhibition of PV interneurons in TBI significantly increases OPG mRNA density compared to TBI alone (Sal-T vs PSEM-T; 22.24 ± 4.43 vs 31.77 ± 4.10). Note that all animals express the PSAM chemogenetic construct (green) but only the PSEM + groups receive the agonist. Small datapoints depict individual neurons, red datapoints depict average per animal (N = 3, used for statistics).Data are shown as mean ± SD *p < 0.05; **p < 0.01