Fig. 2

Persistent increase in CD45^high macrophages after degeneration. A Flow cytometry gating strategy. CD45-IV (intravenous anti-CD45 antibody) was used to exclude immune cells circulating in the retinal vasculature at tissue collection. B Quantification of CD45^low macrophages, which transiently increase in number during degeneration and then return to baseline. C Quantification of CD45^low CD11b⁺ Cx3cr1⁺ Ly6C^low MHCII^low CD45-IV⁻ microglia, which also transiently increase in number during degeneration and then return to baseline. D Quantification of CD45^high macrophages, which transiently increase in number then decrease, but do not fully return to baseline numbers. E CD45^high Ly6C^high cells (red) increased rapidly following light onset, and then returned to baseline by Day 5. In contrast, CD45^high Ly6C^low cells (blue) increased in number more slowly and did not fully return to baseline. n = 2–6 retinas per timepoint from the following numbers of mice: 0 days (n = 2), 1 day (n = 3), 3 days (n = 3) 4 days (n = 2), 5 days (n = 2), 7 days (n = 2), 10 days (n = 2), and 20 days (n = 2). Mean ± SE where n > 3 retinas