Fig. 5

Variations in microglial density and distribution in the DH versus VH CA1 stratum radiatum. Representative brightfield images at a ×20 magnification illustrating IBA1⁺ microglia in the DH (A) and VH (B) CA1 SR. The DH had a higher microglial density compared to the VH (C). Accordingly, the NND was decreased (D) and the spacing index also differed between the two hippocampal poles (E). Data are expressed as mean ± S.E.M. Statistical significance was assessed using a paired Student’s t-test (n = 6 mice). *p-value < 0.1, **p-value < 0.01, and ***p-value < 0.001. a.u.: arbitrary units; DH: dorsal hippocampus; Lac-mol: stratum lacunosum-moleculare; NND: nearest neighbor distance; Rad: stratum radiatum; S.E.M.: standard error of the mean; SP: pyramidal cell layer; VH: ventral hippocampus