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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: The upregulation of NLRP3 inflammasome in dorsal root ganglion by ten-eleven translocation methylcytosine dioxygenase 2 (TET2) contributed to diabetic neuropathic pain in mice

Fig. 2

NLRP3 inflammasome was increased in STZ-diabetic mice DRG and was involved in pain sensitivity. A Representative Western blot bands for NLRP3 and α-tubulin at four weeks post-STZ injection in control and DNP group. B Statistical analysis for NLRP3 and α-tubulin. n = 7, *p < 0.05, unpaired t-test. C Representative Western blot bands for pro-caspase-1, cleaved-caspase-1, and α-tubulin at four weeks in control and DNP group. D Statistical analysis for pro-caspase-1 and α-tubulin. n.s., not significant. E Statistical analysis for cleaved-caspase-1 and α-tubulin. n = 5, **p < 0.01, unpaired t-test. F Statistical analysis for cleaved-caspase-1 and pro-caspase-1. n = 5, **p < 0.01, unpaired t-test. G Representative Western blot bands for pro-IL-1β, cleaved-IL-1β, and α-tubulin at 4 weeks post-STZ injection in control and DNP group. H Statistical analysis for pro-IL-1β and α-tubulin. n.s., not significant. I Statistical analysis for cleaved-IL-1β and α-tubulin. n = 7, *p < 0.05, unpaired t-test. J Statistical analysis for cleaved-IL-1β and pro-IL-1β. n = 7, **p < 0.01, unpaired t-test. K Intraperitoneal injections of 3 mg/kg and 10 mg/kg MCC950 significantly relieved pain sensitivity in the STZ-diabetic mice, while the same did not happen with 1 mg/kg MCC950 injection. n = 10, **p < 0.01, ***p < 0.001 versus the vehicle group, two-way ANOVA with Bonferroni post hoc test. L Intraperitoneal injection of 10 mg/kg MCC950 did not affect the behavior of control mice, n = 10–11, two-way ANOVA with Bonferroni post hoc test. n.s., not significant. M Intrathecal injections of 0.5 mM and 5 mM MCC950 significantly relieved pain sensitivity in the STZ-diabetic mice, while the same did not happen for 0.05 mM MCC950 injection. n = 10–11, *p < 0.05, **p < 0.01, ***p < 0.001 versus the vehicle group, two-way ANOVA with Bonferroni post hoc test. N Intrathecal injection of 5 mM MCC950 did not affected the behavior of control mice. n = 10 –11, two-way ANOVA with Bonferroni post hoc test. n.s., not significant. O Intraperitoneal injection of 5 mg/kg of CY-09 significantly relieved pain sensitivity in the STZ-diabetic mice. n = 10, ***p < 0.001 versus the vehicle group, two-way ANOVA with Bonferroni post hoc test. Experiments were repeated at least 3 times independently

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Journal of Neuroinflammation

ISSN: 1742-2094