Fig. 3

Genomic DNA in the STZ-diabetic mice DRG was demethylated. A Genomic DNA 5mC was examined in both control and DNP group. Methylene blue staining was performed to maintain the quality of the DNA samples when transferred to membrane blots. B Statistical analysis for 5mC. n = 3, unpaired t-test. n.s., not significant. C Genomic DNA 5hmC was examined in control and DNP group, and methylene blue staining was performed to maintain the quality of the DNA samples when transferred to membrane blots. D Statistical analysis for 5hmC. *p < 0.05, n = 5, unpaired t-test. E Genomic DNA 5fC was examined in control and DNP group, and methylene blue staining was performed to maintain the quality of the DNA samples when transferred to membrane blots. F Statistical analysis for 5fC. *p < 0.05, n = 4, unpaired t-test. G Genomic DNA 5caC was examined in control and DNP group, and methylene blue staining was performed to maintain the quality of the DNA samples when transferred to membrane blots. H Statistical analysis for 5caC. **p < 0.01, n = 4, unpaired t-test. I Volcano plot of DNP versus control mice DRG. n = 3. J Heatmap analysis of the differently expressed mRNAs in DNP versus control mice DRG. n = 3. K KEGG pathway enrichment analysis revealed the top 20 signaling pathways. L Representative Western blot bands for TXNIP and α-tubulin at four weeks in control and DNP group. M Statistical analysis for TXNIP and α-tubulin. n = 6, **p < 0.01, unpaired t-test. Experiments were repeated at least 3 times independently except the RNA-sequencing experiment