Fig. 4

TET2 was upregulated in the DRG neurons of STZ-diabetic mice. A Representative Western blot bands for TET2 and α-tubulin at different time points in control and DNP group. B Statistical analysis of the relative band density of TET2. n = 5, *p < 0.05, one-way ANOVA with Tukey’s post hoc test. C Representative Western blot bands for TET1 and α-tubulin at different time points in control and DNP group. D Statistical analysis of the relative band density of TET1. n = 4, n.s., not significant, one-way ANOVA with Tukey’s post hoc test. E Representative Western blot bands for TET3 and α-tubulin at different time points in control and DNP group. F Statistical analysis of the relative band density of TET3. n = 4, n.s., not significant, one-way ANOVA with Tukey’s post hoc test. G Double staining of TET2 and NeuN. H Double staining of TET2 and NF200. I Double staining of TET2 and CGRP. Scale bar = 50 µm. Arrows indicate the representatives of double-stained cells. J The ratio of NeuN⁺, NF200⁺, CGRP⁺ cells in TET2⁺ cells. n = 3. K The mean density of TET2 in nucleus was higher compared to cytoplasm. n = 90 cells, n = 3 mice, ***p < 0.001, unpaired t-test. Experiments were repeated at least 3 times independently