Fig. 6

High glucose upregulated Txnip expression in mouse primary DRG cultures. A–E The mRNA level of TET2 (A), Txnip (B), NLRP3 (C), Caspase-1 (D), and IL-1β (E) in mouse primary DRG cultures under different treatments. n = 3, *p < 0.05, **p < 0.01 versus control; ^#p < 0.05, ^##p < 0.01 versus MT; n.s., not significant, one-way ANOVA with Tukey’s post hoc test. F–H TET2-siRNA or control siRNA was incubated with mouse primary DRG neurons cultured in high glucose (HG) condition, and the mRNA expression of TET2 (F), Txnip (G), and NLRP3 (H). n = 3, *p < 0.05 versus HG + Scr-siRNA; ^#p < 0.05 versus HG + Vehicle; n.s., not significant; one-way ANOVA with Tukey’s post hoc test. Experiments were repeated 3 times independently