Fig. 6
From: Hydrogen attenuates postoperative pain through Trx1/ASK1/MMP9 signaling pathway
Hydrogen acted on the expression of Trx1/ASK1/MMP9 pathway proteins in vitro. The phosphorylation and expression of ASK1 (A), p38 (B), and JNK (C) were measured using western blot in BV-2 cells after NQDI1 treatment. MMP-9 activity and expression (D, E), and IBA-1 expression (F) were measured using western blot and zymography in BV-2 cells after NQDI1 treatment. The activity of MMP-9 (G) and the expression of MMP-9 (H) and Trx1 (I) were measured after H2 treatment. Phosphorylation and expression of ASK1 (J), p38 (K), and JNK (L), and the expression of IBA-1 (M) were measured using western blot after H2 treatment. N Representative images showing NF-kB translocation from the cytosol to the nucleus after different treatments in BV-2 cells. Scale bar: 40 µm; n = 4. Cells are collected and analyzed 24 h after H2 treatment. Significant differences are revealed following one-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001 vs. sham; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. LPS group; $P < 0.05, $$P < 0.01, $$$P < 0.001 vs. LPS + H2 group; Bonferroni post hoc tests)