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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Fecal microbiota transplantation and replenishment of short-chain fatty acids protect against chronic cerebral hypoperfusion-induced colonic dysfunction by regulating gut microbiota, differentiation of Th17 cells, and mitochondrial energy metabolism

Fig. 8

FMT and SCFAs treatment inhibit CCH-induced inflammatory IL-17 pathways in colon. A Representative western blot for TGFβ1, IRF4, RORγT, NLRP3, VCAM1, p-STAT3, STAT3 and GAPDH in colon. B Relative optical density analysis for TGFβ1, IRF4, RORγT, NLRP3, VCAM1, p-STAT3, STAT3 and GAPDH in colon (n = 4 per group). C The protein–protein interaction network downloaded from the STRING database indicated the interactions among the candidate genes. D Representative immunoprecipitation (IP) for IRF4, STAT3 and GAPDH in colon. (E) Relative ratio of STAT3 in IP complex and STAT3 in whole cell lysates in colon (n = 3 per group). The relative abundance of STAT3 in IP complex is determined by western blotting, and compared to the level of STAT3 in whole cell lysates. Data are expressed as mean ± SD. *P < 0.05 vs. sham group. #P < 0.05 vs. BCCAo group

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Journal of Neuroinflammation

ISSN: 1742-2094