Fig. 3

Effect of FGFR1-3 inhibitors BGJ398 and AZD4547 on chemokine and cytokine expression from primary rhesus microglia. Primary rhesus microglia were pretreated with various concentrations of BGJ398 (a) or AZD4547 (b) for 1 and a half to two hours prior to treatment with B. burgdorferi. DMSO was included as the solvent control for drug treatments. After 24 h, supernatants were collected and analyzed for IL-6, CXCL8 and CCL2 by multiplex ELISA. Three-four experiments on microglia derived from 3 tissues were conducted for BGJ398, while 3 experiments from microglia derived from 3 tissues were carried out with AZD4547. One of the experiments is shown for each. Bar represents standard deviation. All statistical comparisons are with B. burgdorferi + DMSO. *p < 0.05, **p < 0.01, and ***p < 0.001