Fig. 5

Fully active differentiated neurons can be obtained after 66 days of monoculture. A The resting membrane potential was significantly higher than the theoretical value (− 65 mV, red dotted line) both at 28 and 66 days. The RMP at 66 days was significantly lower compared to 28 days. B Representative traces of firing events after injecting increasing current in the patching pipette. C The frequency of sEPSCs was significantly higher at 66 days. D Representative traces of gap-free recordings. The red arrowheads indicate the position of EPSCs (Results are shown as mean ± S.E.M.; 28 days: n = 8 cells out of one batch, 66 days: n = 18 cells out of two batches; ^####p < 0.0001: significantly different from − 65 mV; **p < 0.01, ****p < 0.0001: significantly different from the 28-day condition)