Fig. 6

Astrocytes affect the frequency, but not the amplitude, of both sEPSCs and mEPSCs. A Both astrocytes and ACM increased the frequency of sEPSCs compared to control neuronal monocultures. B The amplitude of sEPSCs was not affected by the presence of astrocytes or ACM. D Under TTx 0.5 µM treatment, only the ACM increased the frequency of mEPSCs compared to neuronal monocultures. E The amplitude of mEPSCs was not affected by the presence of astrocytes or ACM. C, F Representative traces of gap-free recordings. Red arrowheads indicate the position of EPSCs. (Results are shown as mean ± S.E.M.; neurons: n = 23 cells out of two batches, neurons + astrocytes: n = 9 cells out of one batch, neurons + ACM: n = 17 cells out of two batches; **p < 0.01, ****p < 0.0001: significantly different from neuronal monocultures)