Fig. 1

Effects of TNEA and its composing acupoints on spatial learning memory and the levels of APP/CTFs in 5xFAD mice. A Representative moving patterns of mice in the probe test. B Quantification of the proportion of the distance traveled in the target quadrant and platform location crossed (mean ± SEM, n = 10–14). *p < 0.05, **p < 0.01, ns (not significant, p > 0.05) vs. 5xFAD group, analyzed by one-way ANOVA. C Representative Western blots showed the levels of full-length APP (Fl-APP) and carboxy-terminal fragments (CTFs) in the hippocampi (HI) of mice from each group. D Data are quantified as mean ± SEM (male, n = 5–7). **p < 0.01, ***p < 0.001, ns (not significant, p > 0.05) vs. 5xFAD group, analyzed by analyzed by unpaired t-test. E Representative Western blots showed the levels of Aβ (~ 5 kDa, detected by 6E10 antibody) in the HI of mice from each group. Another batch of blots used for quantification is shown in Additional file 1: Fig. S1C. F Data are quantified as mean ± SEM (male, n = 4–7). *p < 0.05, ***p < 0.001, ns vs. 5xFAD group, analyzed by unpaired t-test and one-way ANOVA