Fig. 7

TNEA activates TFE3 in the hippocampi of 5xFAD mice. A, B Representative Western blots showed the levels of total TFE3 (A) and cytosolic (Cyt) /nuclear (Nuc) levels of TFE3 (B) in the hippocampi (HI) of mice from each group. GAPDH and H3F3A (H3 histone) were used as cytosolic and nuclear loading controls, respectively. C–E Quantitation results of A and B. Data are quantified as mean ± SEM (male, n = 6). *p < 0.05, ns (p > 0.05) vs. 5xFAD group analyzed by unpaired t-test or unpaired t-test with Welch's correction. F–G Representative IHC staining images of TFE3 (green) and nuclei (Hoechst, blue) in the hippocampal CA1 (F) and CA3 (G) of mice from each group. Scale bar: 100 μm. 4× magnified images were shown for the selected area. H, I Images were processed using ImageJ to quantify the average area of TFE3, (divided by the area of Hoechst). Data are quantified as mean ± SEM (male, n = 5) and analyzed by unpaired t-test. *p < 0.05 vs. 5xFAD group