Fig. 6

Inhibiting ACAT1 decreases retinal neovascularization (RNV) and avascular area (AVA) during OIR. a–e OIR mice were treated with the ACAT1 inhibitor K604 or vehicle from P7 to P16. Eyes were enucleated at P17 and retinal vessels were visualized by IB₄ labeling of retinal flat mounts. a–c Areas of RNV (white highlighted areas) and AVA (yellow outline) were significantly reduced by treatment with K604. n = 10–11, scale bar = 300 μm. d, e K604 treatment increased vascular sprouting as shown by increased numbers of tip cells with filopodia, as compared to vehicle treated OIR retinas. n = 7–8, scale bar = 40 µm. f–h OIR mice were treated with the ACAT1 inhibitor K604 or vehicle from P12 to P16. Eyes were enucleated at P17 and retinal vessels were visualized by IB₄ labeling. Treatment with K604 significantly reduced the formation of RNV (white highlighted areas), but not the AVA (yellow outline). n = 10–11, scale bar = 300 μm. i, j OIR mice were treated with the ACAT1 inhibitor K604 or vehicle from P7 to P11. Eyes were enucleated at P12 and retinal vessels were visualized by IB₄ labeling. Treatment with K604 significantly reduced the AVA (yellow outline). n = 6–7, scale bar = 300 μm