Fig. 2
Vdr conditional knockout in microglia/macrophages worsens poststroke functional outcome. A An overview of the experimental design. B Sensorimotor deficits were evaluated using the mNSS score (top left), corner turning test (top right), foot fault test (bottom left), and Rotarod test (bottom right) in Vdrf/f control and Vdr-cKO mice 1, 3 and 7 days after MCAO (n = 10 per group). C TTC staining of brain sections from control and Vdr-cKO mice 3 days after MCAO. Four representative rostro-caudal brain sections are displayed (n = 6 per group). Scale bar, 5 mm. D TUNEL immunostaining indicates the number of apoptotic cells in the peri-infarct area of ischemic brains of indicated groups (n = 5 per group). Scale bar, 100 µm. E FACS analysis of brain apoptotic cells of control and Vdr-cKO mice using an Annexin-PI kit (n = 6 per group). Each symbol represents one mouse. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, Vdr-cKO versus control group by two-way repeated measures ANOVA with Greenhouse–Geisser correction followed by Sidak's post hoc test for B, and two-tailed Student’s t test for C, D, and E