Fig. 1

Wnt/β-catenin pathway activity in autoimmune uveitis. a Schematic of the clinical evaluation of EAU models (n = 10). b Detection of active β-catenin and total β-catenin levels in spleens from WT mice and mice with EAU on D1, D5, D12, D18, and D25 post-immunization using Western blotting (n = 6). c Expression of Wnt target genes, Lef1, Sox9, Axin2, and Tcf1, in spleens from WT mice and mice with EAU, at D1, D5, D12, D18, and D25 post-immunization, quantified using qPCR (n = 5). d Active β-catenin and total β-catenin levels in the spleens from WT mice and mice with EAU, at D1, D2, D3, D4, and D5 post-immunization, detected using Western blotting (n = 6). e Expression of active β-catenin in CD11c⁺ DCs, B220⁺ B cells, and CD3⁺ T cells in the spleens of WT mice and mice with EAU at D3 and D12 post-immunization, detected using FACS (n = 3). f Active β-catenin and total β-catenin levels in the retinas from WT mice and mice with EAU at D1, D5, D12, D18, and D25 post-immunization, detected using Western blotting (n = 5). g Expression of Wnt target genes, Lef1, Sox9, Axin2, and Tcf1, in retinas from WT mice and mice with EAU at D1, D5, D12, D18, and D25 post-immunization, quantified using qPCR (n = 5). h β-Galactosidase reporter gene expression is illustrated in blue color in the spleen, liver, kidney, and eyes of Wnt reporter mice (Axin2-LacZ) post-immunization on D3 and D12 detected using β-galactosidase staining (n = 3). i Expression of active β-cateninin in CD11c⁺ DCs, CD3⁺ T cells, and B220⁺ B cells from the PBMCs of acute VKH patients and healthy controls detected using FACS. j Mean fluorescence intensity (MFI) was measured from VKH patients (n = 10) and healthy controls (n = 10). Data are presented as mean ± SD. NS > 0.05, *p < 0.05, **p < 0.01