Fig. 4

Effects of PTX treatment on DCs and T cells in vitro. a–c CD11c⁺ DCs were isolated from WT mice and stimulated with IRBP (20 µg/ml), CFA (25 µg/ml), and PTX (5 ng/ml) for 24 h in culture medium. a Expression levels of active β-catenin detected using FACS (n = 3). b, c Expression levels of inflammatory factors expressed in or secreted by CD11c⁺ DCs detected using qPCR or ELISA (n = 5). d, e CD11c⁺ DCs from the spleens of WT mice were stimulated with PBS or PTX for 8 h and then co-cultured with CFSE-labeled naïve OT-II T cells at 1:2 or 1:5 ratios for 4 days in the presence of OVA peptide. d Representative FACS plot showing CFSE-labeled naïve OT-II T cell proliferation stimulated by PBS- or PTX-treated DCs. e The percentages of CFSE-labeled proliferating T cells (n = 3). f, g CD11c⁺ DCs from the spleens of WT mice were stimulated with PBS or PTX for 24 h and then co-cultured with naïve OT-II T cells in a 1:2 ratio in the presence of OVA peptide for 5 days. f Representative FACS plot showing IFN-γ⁺ and IL-17A⁺ CD4⁺ T cell differentiation stimulated by PBS- or PTX-treated DCs. g The percentages of Th17 and Th1 differentiated cells (n = 3). h, i CFSE-labeled T cells were stimulated with CD3/28 and treated with PBS or PTX for 96 h. h Representative FACS plot showing CFSE-labeled naïve OT-II T cell proliferation following treatmeant with PBS or PTX. i The percentages of CFSE-labeled proliferating T cells (n = 3). j, k CFSE-labeled T cells were cultured under Th1 or Th17 differentiation conditions and treated with PBS or PTX for 96 h. j Representative FACS plot showing naïve OT-II T cell differentiation following treatment with PBS or PTX. k The percentages of differentiated T cells (n = 3). Data shown are representative of thre independent experiments. Data are mean ± SD. NS, p > 0.05, *p < 0.05, **p < 0.01