Fig. 5

Effects of conditional β-catenin knockout in DCs on EAU pathogenesis. β-cat^fl/fl and β-cat^DC−/− mice were immunized with IRBP-CFA and intraperitoneally injected with PTX or PBS on day 0 and day 2. a–c The fundus digital image, clinical scores (n = 10), and histological staining (n = 5) of β-cat^fl/fl IRBP-CFA-PBS-treated mice, β-cat^DC−/− IRBP-CFA-PBS-treated mice, β-cat^fl/fl IRBP-CFA-PTX-treated mice, and β-cat^DC−/− IRBP-CFA-PTX-treated mice. d Histopathological scores for EAU in β-cat^fl/fl IRBP-CFA-PBS-treated mice, β-cat^DC−/− IRBP-CFA-PBS-treated mice, β-cat^fl/fl IRBP-CFA-PTX-treated mice, and β-cat^DC−/− IRBP-CFA-PTX-treated mice at day 21 post-immunization (n = 5). e Total number of CD4⁺ T lymphocytes infiltrating the eye on day 21 post-immunization (n = 8). f Expression of IFN-γ and IL-17A in eye-derived CD4⁺ T cells from β-cat^fl/fl PBS-treated mice, β-cat^DC−/− PBS-treated mice, β-cat^fl/fl PTX-treated mice, and β-cat^DC−/− PTX-treated mice detected using FACS at D21 post-immunization. g The percentages of IL17⁺ cells and IFN-γ⁺ cells in eye-derived CD4⁺ T cells from β-cat^fl/fl PBS-treated mice, β-cat^DC−/− PBS-treated mice, β-cat^fl/fl PTX-treated mice, and β-cat^DC−/− PTX-treated mice (n = 3). Data are representative of three independent experiments. Data are mean ± SD. NS, p > 0.05, *p < 0.05, **p < 0.01