Fig. 6

Effect of PTX pretreatment and BIO treatment on EAU development. a Diagram of the time of EAU induction and PTX and BIO administration. b EAU clinical scores of PTX-pre-treated mice, control mice, and BIO-pretreated mice recorded daily from day 6 to day 25 post-immunization (n = 6). c Representative fundus image of PTX-pre-treated mice, control mice, and BIO-pre-treated mice on day 21 after immunization. d Pathological representatives of hematoxylin and eosin (H&E) staining of the eye sections from PTX-pre-treated mice, control mice, and BIO-pre-treated mice on day 21 after immunization. e Histopathological scores for EAU in PTX-pre-treated mice, control mice, and BIO-pre-treated mice on day 21 post-immunization (n = 5). f Total number of CD4⁺ T lymphocytes infiltrating the eye on day 21 post-immunization (n = 6). g Expression of IFN-γ and IL-17A in eye-derived CD4⁺ T cells from PTX-pre-treated mice, control mice, and BIO-pre-treated mice detected using FACS on D21 post-immunization (n = 3). h The percentages of IL-17⁺ cells and IFN-γ⁺ cells in eye-derived CD4⁺T cells from PTX-pre-treated mice, control mice, and BIO-pre-treated mice (n = 3). Data are representative of three independent experiments. Data mean ± SD. NS > 0.05, *p < 0.05, **p < 0.01