Fig. 3

Experimental overview. (1) Experimental model of ischemia with blood collections prior to, or 7 and 28 days following injection of saline or Endothelin-1 into the dorsal striatum (2) Isolation of plasma and storage at − 80 °C until further analysis (3) 10 μL of plasma is incubated with target antibody for 30 min prior to dilution with PBS and nanoflow cytometry analysis (4) EVs pass through lasers with wavelengths of 405 nm, 488 nm and 638 nm and detectors measure SALS, LALS and fluorescent light scatter (5) Size and fluorescent gating are used to generate outputs of the target EV population. Figure created with Biorender.com