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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Septin-3 autoimmunity in patients with paraneoplastic cerebellar ataxia

Fig. 1

Immunofluorescence staining of central nervous tissues with patient sera. Cryosections of rat hippocampus, primate cerebellum and murine encephalon were incubated with patient sera (A, B Patients 1–3, 1:100; C, D Patient 1, 1:100) in the first step, and with Alexa Fluor 488 labeled goat anti-human IgG in the second step (green). A granular staining of the molecular layer (ml) was obtained on hippocampus and cerebellum (A, B). Additionally, a blotchy fluorescence of the granular layer was observed on cerebellum (B). On hippocampus, staining of the outer molecular layer (oml) is more intense compared to the inner molecular layer (iml) (A). On murine encephalon (C), the strongest reactivity was detected in the molecular layer of the cerebellum (D). Nuclei were counterstained by incubation with TO-PRO-3 iodide or DAPI (blue). (Scale bar: A, B 100 µm, C 1000 µm, D 250 µm.)

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