Fig. 3

Tregs depletion aggravates inflammatory responses and pyroptosis of microglia in LPC-induced demyelination. A Expressions of mRNA of inflammatory cytokines and inflammasomes in different groups. N = 3–5 per group. Two-way ANOVA followed by Bonferroni’s test was applied. B–H Representative images of western blot for pyroptosis-related proteins in sham and LPC group treated with PBS and DT (B), and corresponding statistical analysis of protein expressions (C–H). N = 6/group. I–L Representative confocal double-labeling images of Iba1with caspase-1 (I), GSDMD (J), NLRP3 (K) and IL-1β (L) in CC and corresponding quantifications of double-positive cells among different groups. CC in sham group and lesions in LPC group were indicated by dashed line. Magnified images were shown and enlarged 3D views were recontributed by Imaris. Scale bars were 50 μm in confocal images and 5 μm in 3D images, respectively. N = 5/group. Two-way ANOVA with Tukey’s post hoc test was used. *P < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001