Fig. 4

Microglial BMAL1 deficiency decreases C1q and increases C3 in aged CA1 hippocampus. A Representative images of C1q (green), PSD95 (magenta) and CD68 (red) expression in the hippocampal CA1 region of aged CD11b^cre and CD11b^cre;Bmal1^lox/lox mice (18–20 months). Scale bar, 10 µm. White arrow shows colocalization of C1q, PSD95 and CD68 present in aged CD11b^cre that is absent in CD11b^cre;Bmal1^lox/lox mice. B Mean fluorescence intensity (MFI) of C1q, PSD95 and CD68 (n = 6/group). C Schematic illustrating C1q activation of C3. D Representative confocal images of C1q (green), PSD95 (magenta) and C3 (red) expression in the CA1 hippocampal area in aged CD11b^cre and CD11b^cre;Bmal1^lox/lox mice. Scale bar, 20 µm. E MFI of C3 in aged CD11b^cre and CD11b^cre;Bmal1^lox/lox mice (n = 6/group). Data are represented as the mean ± SEM. P-values were calculated using two-tailed Student’s t-test