Fig. 2

Bone marrow and blood fibrocytes in young (2.5-month) and aged (15–16-month) mice. A Gating strategies of blood fibrocytes. The gating strategies were based on relevant isotype controls. CD45⁺Collagen-1⁺ cells were considered circulating fibrocytes. B, C Percentage of CD45⁺collagen1⁺ fibrocytes in bone marrow (B) and blood (C) from young and aged mice under normal conditions. D, E Percentage of CD45⁺collagen1⁺ fibrocytes in bone marrow (D) and blood (E) from young and aged mice with/without subretinal fibrosis (SRF). Mean ± SD, n = 4–5, **p < 0.01, ***p < 0.001, Student t test. F, G Representative confocal images of blood cells stained for CD45 (green) and collagen-1 (red) from young (F) and aged mice (G) with subretinal fibrosis. Cell nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI, blue), Scale bar = 20 um. (H) Magnified area of box in (G) showing CD45⁺collagen1⁺ cells (arrows) and CD45⁺collagen1⁻ cells (asterisks). Scale bar = 5 µm. I Percentage of CD45⁺collagen1⁺ fibrocytes detected by immunocytochemistry in the bone marrow and blood from young and aged mice with subretinal fibrosis. Mean ± SD, n = 3, *p < 0.05, Student t test