Fig. 6

NOX contributes to Mac1-mediated NLRP3 inflammasome activation in microglia intoxicated with P + M. A Representative blots of NLRP3, active caspase-1 and mature IL-1β in BV2 microglia treated with P + M for 24 h and the quantification of the blots. The concentrations of P + M were 5 + 0.3 and 10 + 0.6 μM, which were chosen based on previous report [24]. B Representative blots of active caspase-1 and mature IL-1β in P + M-treated BV2 microglia with or without RGD (10 μM) pretreatment (30 min) and the quantification of density of these blots. C Representative blots of active caspase-1 and mature IL-1β in P + M-treated BV2 microglia with or without anti-Mac1 Ab (1 μg/ml) pretreatment (30 min) and the quantification of density of these blots. D Representative blots of active caspase-1 and mature IL-1β in P + M-treated BV2 microglia with or without apocynin (0.25 mM) pretreatment (30 min) and the quantification of density of these blots. E Representative blots of active caspase-1 and mature IL-1β in P + M-treated BV2 microglia with or without RGD and PMA (100 ng/ml) pretreatment (30 min) and the quantification of density of these blots. F Representative blots of active caspase-1 and mature IL-1β in NOX1 and NOX2 silenced BV2 microglia treated with P + M and the quantification of density of these blots. G Representative blots of phosphorylated and nonphosphorylated ERK1/2, p38, JNK and PAK1 in P + M-treated BV2 microglia with or without apocynin pretreatment (30 min) and the quantification of density of these blots. n = 3; *p < 0.05, **p < 0.01