Fig. 7

Glybenclamide suppresses microglial M1 polarization, neurodegeneration, synaptic loss and α-synuclein phosphorylation as well as spatial learning deficits in P + M-injected mice. A Representative blots of NLRP3, active caspase-1 and mature IL-1β in the hippocampus. B Quantification of the blots. C Representative pictures of Iba-1 staining in the hippocampus and quantification of Iba-1 optical density. n = 3. D, E mRNA levels of M1 (D) and M2 (E) genes. n = 6. F Quantification of Neu-N + cells. G Quantification of PSD-95 optical density. H Representative blots and quantification of Neu-N, PSD-95, TH, total and Ser129-phosphorylated α-synuclein in mice. n = 6. I Escape latency, J traveled distance and (K) swimming speed of mice. n = 15–16; *p < 0.05, **p < 0.01; Scale bar = 50 μm