Fig. 3

Inhibition of lipid droplet accumulation induced by ZIKV decreases viral replication in neuroblastoma cells. A Representative images of SH-SY5Y cells 48 h after ZIKV infection treated with 50 µM DGAT-1 inhibitor (A922500) and stained with Oil Red O (red) and DAPI (blue) for nuclei. The scale bar represents 10 µm in range. B Quantification of the fluorescent area per cell in each group. C Real-time PCR for PLIN-2 after 24 h of ZIKV infection and treatment with a DGAT-1 inhibitor. D Effect of the DGAT-1 inhibitor on ZIKV replication at 48 hpi. E Images of SH-SY5Y cells after 48 h of ZIKV infection and the ds-RNA stained with J2 antibody (green), the lipid droplets were stained with LipidTox (Red) and nuclei with DAPI (blue). The scale bar represents 10 µm in range. F Quantification of LD pixels per cell. G Quantification of ds-RNA pixels per cell. Data information: In B–D, F, G), data are presented as the means ± SEMs of three independent experiments. *P < 0.05 mock- versus ZIKV-infected cells. #P < 0.05 ZIKV-infected cells versus A922500 treatments