Fig. 1

Minor changes in ISG15, OAS1 and PKR protein expression between TMEV- and mock-infected mice. A Hippocampus of SJL and C57BL/6 mice at 4 days post-infection (dpi). Immunohistochemistry using the avidin–biotin-peroxidase complex method with the chromogen 3′3-diaminobenzidine and Mayer’s hematoxylin counterstaining. B Morphometric analysis of immunohistochemically stained coronal brain sections cut at the level of the hippocampus (1 complete section per mouse evaluated; n = 6). Note significantly higher expression of ISG15 in TMEV-infected C57BL/6 mice at 4 and 14 dpi as well as in TMEV-infected SJL mice at 7 dpi compared to the respective mock-infected mice. No significant influence of TMEV infection on OAS1 and PKR protein expression. Kruskal–Wallis tests with Dunn’s multiple comparisons tests: *p < 0.05. Shown are all data points with means. Each data point represents the immunopositive area of one mouse