Fig. 3

IFNAR signaling of microglia but not of neuroectodermal cells has a significant impact on the number of perivascular mononuclear cells in the cerebrum at 4 days after TMEV-BeAn infection. A Histological lesions in the hippocampus of TMEV-infected IFNAR^−/−, NesCre^±IFNAR^fl/fl, GFAPCre^±IFNAR^fl/fl, Syn1Cre^±IFNAR^fl/fl and Cre^−/−IFNAR^fl/fl mice as well as tamoxifen (Tam)-treated Sall1Cre^ER± mice and Sall1Cre^ER−/− control mice. Hematoxylin and eosin staining. B Semiquantitative analysis of coronal brain sections cut at the level of the hippocampus (1 complete section per mouse evaluated) revealed a higher number of perivascular mononuclear cells in Tam-treated Sall1CreER^± mice (n = 6) compared to Sall1CreER^−/− control mice (n = 9), whereas no significant differences were found between Cre^−/−IFNAR^fl/fl mice (n = 7) and IFNAR^−/− (n = 6), NesCre^±IFNAR^fl/fl (n = 10), GFAPCre^±IFNAR^fl/fl (n = 9) and Syn1Cre^±IFNAR^fl/fl mice (n = 10). Mann–Whitney tests using the Bonferroni correction for multiple comparisons: ** p = 0.003. Shown are all data points with means. Each data point represents the semiquantitative score of one mouse