Fig. 5
IFNAR deficiency of astrocytes affects Il6 and Il10 mRNA transcript levels in the cerebrum at 4 days after TMEV-BeAn infection. RT-qPCR showed a downregulation of Isg15 and Eif2ak1 and an upregulation of Tnfa and Il1b mRNA transcripts in IFNAR−/− mice (n = 6) compared to Cre−/−IFNARfl/fl mice (n = 5). NesCre±IFNARfl/fl mice (n = 8) demonstrated an upregulation of Ifnb1, Tnfa, Il6, Il10, Il12b and Ifng mRNA transcripts compared to Cre−/−IFNARfl/fl mice. Il6 and Il10 mRNA transcripts were also higher in GFAPCre±IFNARfl/fl mice (n = 8) than in Cre−/−IFNARfl/fl mice, whereas no significant differences were found between Syn1Cre±IFNARfl/fl (n = 6) and Cre−/−IFNARfl/fl mice. Only Ifng mRNA transcripts were increased in tamoxifen (Tam)-treated Sall1CreER± mice (n = 6) compared to Sall1CreER−/− control mice (n = 8). The mRNA copy numbers were normalized using a normalization factor calculated from three housekeeping genes (Gapdh, Actb, Hprt). Mann–Whitney tests using the Bonferroni correction for multiple comparisons: * p < 0.05; ** p < 0.01. Shown are all data points with means. Each data point represents the mRNA copy number of one mouse