Fig. 6

IFNAR deficiency results in increased IFN-α, IFN-β, IL1-β, IL-6, and CXCL-1 protein expression in the cerebrum at 4 days after TMEV-BeAn infection. A bead-based immunoassay demonstrated increased protein levels of IFN-α, IFN-β, IL1-β, IL-6, and CXCL-1 in IFNAR^−/− mice (n = 3) compared to Cre^−/−IFNAR^fl/fl mice (n = 5). IFN-α and IFN-β protein expression was also elevated in NesCre^±IFNAR^fl/fl mice (n = 8). Tamoxifen (Tam)-treated Sall1Cre^ER± mice (n = 6) showed increased CXCL-1 protein levels compared to Sall1Cre^ER−/− control mice (n = 8). GFAPCre^±IFNAR^fl/fl mice (n = 8) and Syn1Cre^±IFNAR^fl/fl (n = 6) lacked statistically significant changes in cytokine (IFN-α, IFN-β, IFN-γ, TNF, IL1-β, IL-6, IL-10, IL-12) and chemokine (CCL-2, CCL-5, CXCL-1, CXCL-10) protein expression. Kruskal–Wallis tests with Dunn’s multiple comparisons tests: *p < 0.05; **p < 0.01. Shown are all data points with means. Each data point represents the protein concentration (Conc.) of one mouse