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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Human umbilical cord-derived mesenchymal stem cell transplantation supplemented with curcumin improves the outcomes of ischemic stroke via AKT/GSK-3β/β-TrCP/Nrf2 axis

Fig. 4

Combined curcumin-hUC-MSC treatment promoted anti-inflammatory phenotype microglia in MCAO mice. A, B Representative images of immunofluorescence analysis for microglia polarization, (A) The microglial cell-specific marker Iba1 (green), pro-inflammatory microglia marker CD86 (red), nuclei (blue); (B) the microglial cell-specific marker Iba1 (green), anti-inflammatory microglia marker Arg1 (red), nuclei (blue). C, D Quantitative analysis of pro-inflammatory (C) and anti-inflammatory (D) microglia in the ipsilateral peri-infarct. E Representative images of flow cytometry analysis for microglia polarization. F–G Quantitative analysis of the percentage of CD86+ cells (F) and CD206+ microglia (G) in the ipsilateral peri-infarct isolated from MCAO mice subjected to different treatments. H, I qRT-PCR analysis for iNOS (H) and CD86 (I) related to pro-inflammatory microglia gene and Arg1 (J) and CD206 (K) related to anti-inflammatory microglia gene. Cur curcumin, scale bars = 20 μm; N = 5; N.S. no significant difference, *P < 0.05, **P < 0.01, ***P < 0.001

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Journal of Neuroinflammation

ISSN: 1742-2094