Fig. 2

Co-expression of KLF4-C3 or KLF4-S100A10 after focal cerebral ischemia. A Images show the dual-IF staining for KLF4 with C3 as well as S100A10 in ischemic penumbra from mice 2, 4 and 7 days after focal cerebral ischemia. Scale bar = 100 µm for the left three columns, Scale bar = 25 µm for Zoom panels. B, C Quantification of KLF4 + C3 + cell number (B) and KLF4 + S100A10 + cell number (C) in the ischemic penumbra. Results are expressed as the mean ± standard deviation of the double-positive events per field of view, and the data were analyzed by one-way ANOVA (n = 8 per experimental group). Note that in the ischemic penumbra, the co-expression of KLF4-C3 or KLF4-S100A10 increased with time and reached a maximum level at day 7 post-ischemia. However, the distribution patterns of their expressions were different, where the high levels of KLF4 was expressed, relatively low levels of C3 were expressed in the ischemic penumbra, and vice versa. However, KLF4 was always co-stained well with S100A10 in the penumbra at days 2, 4 and 7 post-ischemia, especially at day 7, KLF4 co-localized with S100A10 extensively. **P < 0.01, ***P < 0.001 compared with 2-day post-ischemia