Fig. 3

Analysis of RNA-seq. a Representation of the differentially expressed genes represented by the Venn diagram. b Representation of the heatmap represented top differentially expressed genes between CON and CORT groups, n = 3 in each group. c Representation of the heatmap represented top differentially expressed genes between CORT and BBR groups, n = 3 in each group. d Results of qPCR showed that NLRP3 expression in PFC in CORT was higher than in CON and BBR mice (One-way ANOVA, F (2, 6) = 44.11, P = 0.0003; Dunnett’s multiple comparisons test, CORT vs. Control, P = 0.0002, CORT vs. BBR, P = 0.0012, n = 3 in each group). e Results of qPCR showed that caspase-1 expression in PFC in CORT was higher than in CON and BBR mice (One-way ANOVA, F (2, 6) = 28.61, P = 0.0009; Dunnett’s multiple comparisons test, CORT vs. Control, P = 0.0006, CORT vs. BBR, P = 0.0049, n = 3 in each group). f Results of qPCR showed that IL-1beta expression in PFC in CORT was higher than in CON and BBR mice (One-way ANOVA, F (2, 6) = 17.86, P = 0.0030; Dunnett’s multiple comparisons test, CORT vs. Control, P = 0.0018, CORT vs. BBR, P = 0.0397, n = 3 in each group). g Representation of the top associated gene ontology terms between CON and CORT groups. h Representation of the top associated gene ontology terms between CORT and BBR group. i Representation of the top KEGG enrichment pathways between CON and CORT groups. j Representation of the top KEGG enrichment pathways between CORT and BBR group. CON control, CORT corticosterone, BBR berberine, ANOVA analysis of variance, NLRP3 NOD-like receptor thermal protein domain associated protein 3, qPCR quantitative reverse transcription polymerase chain reaction, PFC prefrontal cortex