Fig. 7

Berberine prevents CORT-induced patch-clamp alteration. a Representation of the data from the excitatory neuron of contrl (black), CORT (red), and BBR 200 mg/kg/day (green), which were stimulated by electricity from 50 to 350 pA, b representation of the data from the excitatory neuron, which were stimulated by electricity from 50 to 400 pA and records of the number of spikes (Two-way ANOVA, row factor F (7, 360) = 22.10, P < 0.0001, column factor F (2, 360) = 86.48, P < 0.0001; Tukey’s multple comparisons test, for 150 pA, CORT vs. control, P = 0.0008, CORT vs. BBR, P = 0.0042; for 250 pA, CORT vs. control, P < 0.0001, CORT vs. BBR, P = 0.0021; for 350 pA, CORT vs. control, P < 0.0001, CORT vs. BBR, P = 0.0023, n = 3 in each group). c Representation of the mEPSC of contrl (black), CORT (red), and BBR 200 mg/kg/day (green) d Representation of the amplitude of mEPSC (One-way ANOVA, F (2, 31) = 1.038, P = 0.3662). e Frequency of mEPSC was significantly lower in CORT compared to CON and BBR 200 mg/kg/day (One-way ANOVA, F (2, 31) = 6.972, P = 0.0032; Tukey’s multple comparisons test, CORT vs. control, P = 0.0046, CORT vs. BBR, P = 0.0388, n = 3 in each group). f Representation of the sEPSC of contrl (black), CORT (red), and BBR 200 mg/kg/day (green) g Representation of the amplitude of sEPSC (One-way ANOVA, F (2, 30) = 0.7402, P = 0.4855, n = 3 in each group). h Representation of the the frequency of sEPSC was significantly lower in CORT compared to CON and BBR 200 mg/kg/day (One-way ANOVA, F (2, 32) = 20.70, P < 0.0001; Tukey’s multple comparisons test, CORT vs. control, P = 0.0040, CORT vs. BBR, P < 0.0001, n = 3 in each group). Bar graphs show the mean ± SEM; *P < 0.05, **P < 0.01, and ***P < 0.001. CORT, corticosterone; BBR, berberine; ANOVA, analysis of variance; mEPSC, miniature excitatory postsynaptic current; sEPSC, spontaneous excitatory postsynaptic current