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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Microglia specific deletion of miR-155 in Alzheimer’s disease mouse models reduces amyloid-β pathology but causes hyperexcitability and seizures

Fig. 7

Inducible knock-out of miR-155 in microglia results in increased internalization of excitatory synaptic terminals marked by VGLUT1 in the 5xFAD brain. A Microglia were isolated using ex vivo, then fixed and stained for the pre-synaptic vesicular GABA transporter (VGAT; representative histograms B in and quantification in C), and the pre-synaptic vesicular glutamate transporter 1 (VGLUT1; representative histograms D in and quantification in E). Total levels of internalized VGAT and VGLUT1 were detected with flow cytometry (LSRII). Data were analyzed using FlowJo and R studio, graphs were generated using Prism 9. (Stats: two-way Ordinary ANOVA with multiple comparisons with Tukey’s post hoc corrections, ****p < 0.0001, ***p < 0.001, **p < 0.01)

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Journal of Neuroinflammation

ISSN: 1742-2094