Fig. 6

Clenbuterol reduces T-cell infiltration and rescues behavioral deficits after hα-SYN overexpression in the SN. A Representative images of the SN showing CD3 (red) and TH (white) staining and B confocal close up showing CD3+ cells in contact with TH cell bodies and axons (arrows). C Quantification of CD3+ T cells associated with the SN 4 weeks after rAAV2-control or rAAV2–hα-SYN injection in WT mice treated with saline, Clenbuterol or Xamoterol daily. D Quantification of sensorimotor bias in a corridor task 4 weeks after rAAV2–hα-SYN injection in WT treated with saline or clenbuterol daily. Black horizontal dash line represents the expected % of contralateral explorations in an uninjured WT mouse. Confocal images are shown as maximum intensity projections. Data are shown as mean values ± SEM, each dot represents one mouse, N.S: not significant, *p < 0.05; **p < 0.01; ****p < 0.0001. One-way ANOVA followed by Tukey post hoc test; two-tailed t test. Scale bar = A, B 500 µm, D 20 µm