Fig. 2

ICM ameliorated the abnormal secretion of HMGB1 in the hippocampus of SAE mice. A Representative images of immunofluorescence staining of Iba-1 (red), HMGB1 (green), DAPI (blue) and colocalization in the hippocampus, scale bar = 10 μm; B, C Quantification of the mean gray value of n-HMGB1 in microglia and non-microglia cells in the hippocampus among the four groups. Data are presented as the mean ± SEM (n = 3–4 mice/group). ****P < 0.0001 vs. the indicated groups