Fig. 3

ICM restored the changes in the number and morphology of microglia in the hippocampus of SAE mice. A Representative images of immunofluorescence staining of Iba-1 (green) and DAPI (blue) in the hippocampus, scale bar = 50 μm; B quantification of the number of Iba-1⁺ cells (microglia) in the hippocampus among the four groups; C representative images of immunofluorescence staining of Iba-1 (yellow) and skeletonization of Iba-1⁺ cells (microglia) in the hippocampus, scale bar = 50 μm; D quantification of the average branch length of Iba-1⁺ cells (microglia) in the hippocampus among the four groups; E quantification of the solidity of Iba-1⁺ cells (microglia) in the hippocampus among the four groups. Data are presented as the mean ± SEM (n = 3–5 mice/group). *P < 0.05, ***P < 0.001, ****P < 0.0001 vs. the indicated groups